Detecting Rare Mutations with Heterogeneous Effects Using a Family-Based Genetic Random Field Method.

The genetic etiology of many complex diseases is highly heterogeneous. A complex disease can be caused by multiple mutations within the same gene or mutations in multiple genes at various genomic loci. Although these disease-susceptibility mutations can be collectively common in the population, they...

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Published in:Genetics Vol. 210; no. 2; pp. 463 - 477
Main Authors: Ming Li, Zihuai He, Xiaoran Tong, Witte, John S., Qing Lu
Format: Journal Article
Published: Oxford University Press / USA Oct2018
Online Access:View this record in EBSCOhost
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      dt: Oct2018
      vid: 210
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      pub: Oxford University Press / USA
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        132297959
        10.1534/genetics.118.301266
        132297959
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        atl: Detecting Rare Mutations with Heterogeneous Effects Using a Family-Based Genetic Random Field Method.
      aug:
        au:
          Ming Li
          Zihuai He
          Xiaoran Tong
          Witte, John S.
          Qing Lu
        affil: Department of Epidemiology and Biostatistics, Indiana University at Bloomington, Indiana 47405
      sug:
        subj:
          Mutation Evaluation
          Genetic Techniques Methods
          Human
          Disease Susceptibility
          Sequence Analysis Methods
          Polymorphism, Genetic
          Conceptual Framework
          Phenotype Evaluation
          Alcoholism Familial and Genetic
          Gene Expression
      ab: The genetic etiology of many complex diseases is highly heterogeneous. A complex disease can be caused by multiple mutations within the same gene or mutations in multiple genes at various genomic loci. Although these disease-susceptibility mutations can be collectively common in the population, they are often individually rare or even private to certain families. Familybased studies are powerful for detecting rare variants enriched in families, which is an important feature for sequencing studies due to the heterogeneous nature of rare variants. In addition, family designs can provide robust protection against population stratification. Nevertheless, statistical methods for analyzing family-based sequencing data are underdeveloped, especially those accounting for heterogeneous etiology of complex diseases. In this article, we introduce a random field framework for detecting gene-phenotype associations in family-based sequencing studies, referred to as family-based genetic random field (FGRF). Similar to existing familybased association tests, FGRF could utilize within-family and between-family information separately or jointly to test an association. We demonstrate that FGRF has comparable statistical power with existing methods when there is no genetic heterogeneity, but can improve statistical power when there is genetic heterogeneity across families. The proposed method also shares the same advantages with the conventional family-based association tests (e.g., being robust to population stratification). Finally, we applied the proposed method to a sequencing data from the Minnesota Twin Family Study, and revealed several genes, including SAMD14, potentially associated with alcohol dependence.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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