Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects.
Purpose: Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential of an olive pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g of hydroxytyrosol and its derivatives, to modulate the composition and metaboli...
| Publicado en: | European Journal of Nutrition Vol. 58; no. 1; pp. 63 - 82 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research randomized controlled trial Journal Article |
| Publicado: |
Springer Nature
Feb2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=135451121&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 135451121 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Feb2019 vid: 58 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 135451121 135451121 135451121 10.1007/s00394-017-1572-2 135451121 ppf: 63 ppct: 19 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects. aug: au: Conterno, Lorenza Martinelli, Francesca Tamburini, Matteo Fava, Francesca Mancini, Andrea Sordo, Maddalena Pindo, Massimo Martens, Stefan Masuero, Domenico Vrhovsek, Urska Dal Lago, Claudia Ferrario, Gabriele Morandini, Mario Tuohy, Kieran affil: OlioCRU s.r.l. Research and Development Group, Via Aldo Moro 1, 38062, Arco, TN, Italy sug: subj: Hypercholesterolemia Metabolism Olive Oil Pharmacodynamics Plant Extracts Pharmacodynamics Food Gut Microbiota Drug Effects Ethanol Analogs and Derivatives Human Double-Blind Studies Randomized Controlled Trials Random Assignment Hematologic Tests Fasting Urinalysis Feces Analysis Cardiovascular Diseases Biological Markers Lipoproteins, LDL Cholesterol Drug Effects Phenols Drug Effects Gram-Positive Bacteria Drug Effects Polymerase Chain Reaction RNA In Situ Hybridization, Fluorescence Sex Factors ab: Purpose: Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential of an olive pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g of hydroxytyrosol and its derivatives, to modulate the composition and metabolic activity of the human gut microbiota.Methods: In a double-blind, controlled parallel dietary intervention 62 otherwise healthy hypercholesterolemic (total plasma cholesterol 180-240 mg/dl) subjects were randomly assigned to eat 90 g of olive pomace-enriched biscuit (olive-enriched product, OEP) or an isoenergetic control (CTRL) for 8 weeks. Fasted blood samples, 24-h urine and faecal samples were collected before and after dietary intervention for measurement of microbiota, metabolites and clinical parameters.Results: Consumption of OEP biscuits did not impact on the diversity of the faecal microbiota and there was no statistically significant effect on CVD markers. A trend towards reduced oxidized LDL cholesterol following OEP ingestion was observed. At the genus level lactobacilli and Ruminococcus were reduced in OEP compared to CTRL biscuits. A trend towards increased bifidobacteria abundance was observed after OEP ingestion in 16S rRNA profiles, by fluorescent in situ hybridization and by qPCR. Targeted LC-MS revealed significant increases phenolic acid concentrations in 24-h urine following OEP ingestion and 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid, derivatives of hydroxytyrosol, were elevated in blood. A sex effect was apparent in urine small phenolic acid concentrations, and this sex effect was mirrored by statistically significant differences in relative abundances of faecal bacteria between men and women.Conclusion: Ingestion of OEP biscuits led to a significant increase in the metabolic output of the gut microbiota with an apparent sex effect possibly linked to differences in microbiota makeup. Increased levels of homovanillic acid and DOPAC, thought to be involved in reducing oxidative LDL cholesterol, were observed upon OEP ingestion. However, OEP did not induce statistically significant changes in either ox-LDL or urinary isoprostane in this study. pubtype: Academic Journal doctype: research randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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