Chronic Peripheral Ghrelin Injection Exerts Antifibrotic Effects by Increasing Growth Differentiation Factor 15 in Rat Hearts With Myocardial Fibrosis Induced by Isoproterenol.
This study aimed to investigate the anti-fibrotic effects of ghrelin in isoproterenol (ISO)-induced myocardial fibrosis and the underlying mechanism. Sprague-Dawley rats were randomized to control, ISO, and ISO + ghrelin groups. ISO (2 mg/kg per day, subcutaneous) or vehicle was administered once da...
| Publicado en: | Physiological Research Vol. 69; no. 3; pp. 439 - 451 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Institute of Physiology, Academy of Sciences of the Czech Republic
Jun2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=144706018&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 144706018 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 08628408 1CVW jtl: Physiological Research issn: 08628408 maglogo: N pubinfo: dt: Jun2020 vid: 69 iid: 3 pid: 24634 pub: Institute of Physiology, Academy of Sciences of the Czech Republic artinfo: ui: 144706018 10.33549/physiolres.934183 144706018 ppf: 439 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Chronic Peripheral Ghrelin Injection Exerts Antifibrotic Effects by Increasing Growth Differentiation Factor 15 in Rat Hearts With Myocardial Fibrosis Induced by Isoproterenol. aug: au: Qian REN Ping LIN Qin WANG Bin ZHANG Li FENG affil: Geriatric Department of the Third Hospital of Hangzhou, Hangzhou, China sug: ab: This study aimed to investigate the anti-fibrotic effects of ghrelin in isoproterenol (ISO)-induced myocardial fibrosis and the underlying mechanism. Sprague-Dawley rats were randomized to control, ISO, and ISO + ghrelin groups. ISO (2 mg/kg per day, subcutaneous) or vehicle was administered once daily for 7 days, then ghrelin (100 µg/kg per day, subcutaneous) was administered once daily for the next 3 weeks. Ghrelin treatment greatly improved the cardiac function of ISO-treated rats. Ghrelin also decreased plasma brain natriuretic peptide level and ratios of heart weight to body weight and left ventricular weight to body weight. Ghrelin significantly reduced myocardial collagen area and hydroxyproline content, accompanied by decreased mRNA levels of collagen type I and III. Furthermore, ghrelin increased plasma level of growth differentiation factor 15 (GDF15) and GDF15 mRNA and protein levels in heart tissues, which were significantly decreased with ISO alone. The phosphorylation of Akt at Ser473 and GSK-3β at Ser9 was decreased with ISO, and ghrelin significantly reversed the downregulation of p -Akt and p -GSK-3β. Mediated by GDF15, ghrelin could attenuate ISO-induced myocardial fibrosis via Akt-GSK-3β signaling. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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