One-year dietary supplementation with walnuts modifies exosomal miRNA in elderly subjects.
Purpose: Epidemiological studies and clinical trials support the association of nut consumption with a lower risk of prevalent non-communicable diseases, particularly cardiovascular disease. However, the molecular mechanisms underlying nut benefits remain to be fully described. MicroRNAs (miRNAs) ar...
| Published in: | European Journal of Nutrition Vol. 60; no. 4; pp. 1999 - 2012 |
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| Main Authors: | , , , , , , , , , , , , , , , , |
| Format: | research tables/charts randomized controlled trial Journal Article |
| Published: |
Springer Nature
Jun2021
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=150416109&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 150416109 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Jun2021 vid: 60 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 150416109 146133213 150416109 150416109 10.1007/s00394-020-02390-2 150416109 ppf: 1999 ppct: 13 formats: fmt: @attributes: type: P tig: atl: One-year dietary supplementation with walnuts modifies exosomal miRNA in elderly subjects. aug: au: López de las Hazas, María-Carmen Gil-Zamorano, Judit Cofán, Montserrat Mantilla-Escalante, Diana C. Garcia-Ruiz, Almudena del Pozo-Acebo, Lorena Pastor, Oscar Yañez-Mo, María Mazzeo, Carla Serra-Mir, Mercè Doménech, Monica Valls-Pedret, Cinta Rajaram, Sujatha Sabaté, Joan Ros, Emilio Sala-Vila, Aleix Dávalos, Alberto affil: Laboratory of Epigenetics of Lipid Metabolism, Instituto Madrileño de Estudios Avanzados (IMDEA)-Alimentación, IMDEA Food Institute, CEI UAM+CSIC, Ctra. De Cantoblanco 8, 28049, Madrid, Spain sug: subj: Dietary Supplementation In Old Age Walnut Therapeutic Use Walnut Pharmacodynamics MicroRNA Blood Cell-Free Nucleic Acids Blood Exosomes Analysis Biological Markers Blood Human Descriptive Statistics Randomized Controlled Trials Random Assignment Aged Pretest-Posttest Design Prospective Studies Metabolomics Nanoparticles Aged: 65+ years ab: Purpose: Epidemiological studies and clinical trials support the association of nut consumption with a lower risk of prevalent non-communicable diseases, particularly cardiovascular disease. However, the molecular mechanisms underlying nut benefits remain to be fully described. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression and play a pivotal role in health and disease. Exosomes are extracellular vesicles released from cells and mediate intercellular communication. Whether nut consumption modulates circulating miRNAs (c-miRNAs) transported in exosomes is poorly described. Methods: Cognitively healthy elderly subjects were randomized to either control (n = 110, abstaining from walnuts) or daily supplementation with walnuts (15% of their total energy, ≈30–60 g/day, n = 101) for 1-year. C-miRNAs were screened in exosomes isolated from 10 samples, before and after supplementation, and identified c-miRNA candidates were validated in the whole cohort. In addition, nanoparticle tracking analysis and lipidomics were assessed in pooled exosomes from the whole cohort. Results: Exosomal hsa-miR-32-5p and hsa-miR-29b-3p were consistently induced by walnut consumption. No major changes in exosomal lipids, nanoparticle concentration or size were found. Conclusion: Our results provide novel evidence that certain c-miRNAs transported in exosomes are modulated by walnut consumption. The extent to which this finding contributes to the benefits of walnuts deserves further research. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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