| Sumario: | Pre-transfusion testing to determine patient blood group profiles and detect clinically significant antibodies is critical for transfusion. However, there is no single method for detecting red cell antibodies that all laboratories use in Australia. This study compared four widely used methods to identify the most sensitive and accurate platform. Previously tested, 50 negative and 48 positive stored samples were used in this study. Samples were analysed for the presence of various red cell antibodies using the Ortho glass bead, BioRad gel, Grifols gel column agglutination technologies (CAT) and Immucor Echo Solid Phase Red Cell adherence assay (SPRCA). Results from all platforms were comparable (tetrachoric correlation co-efficient > 95%). There were a few samples with low titre antibodies that could not be detected by all platforms. The results of this study demonstrate that all four platforms perform equally well, however multi centre studies would be useful to further evaluate these technologies, and to compare protocols in various laboratories.
|
|---|