A comparison of column agglutination and solid phase red cell adherence technologies for red cell antibody detection.
Pre-transfusion testing to determine patient blood group profiles and detect clinically significant antibodies is critical for transfusion. However, there is no single method for detecting red cell antibodies that all laboratories use in Australia. This study compared four widely used methods to ide...
| Publicado en: | Australian Journal of Medical Science Vol. 42; no. 4; pp. 115 - 121 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Australian Institute of Medical Scientists
Nov2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154919290&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154919290 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10381643 GD50 jtl: Australian Journal of Medical Science issn: 10381643 maglogo: N pubinfo: dt: Nov2021 vid: 42 iid: 4 pid: 88256 pub: Australian Institute of Medical Scientists place: Milton, Queensland, <Blank> artinfo: ui: 154919290 154919290 154919290 154919290 ppf: 115 ppct: 6 formats: fmt: @attributes: type: P tig: atl: A comparison of column agglutination and solid phase red cell adherence technologies for red cell antibody detection. aug: au: Daniel, Nora Finch, Susan Vanniasinkam, Thiru affil: Path West Pathology, Department of Haematology, Transfusion Medicine Laboratory, Western Australia sug: subj: Agglutination Tests Methods Erythrocytes Analysis Technology, Medical Transfusion Medicine Antibodies Analysis Histocompatibility Testing Methods Human Immunoassay Methods Comparative Studies ab: Pre-transfusion testing to determine patient blood group profiles and detect clinically significant antibodies is critical for transfusion. However, there is no single method for detecting red cell antibodies that all laboratories use in Australia. This study compared four widely used methods to identify the most sensitive and accurate platform. Previously tested, 50 negative and 48 positive stored samples were used in this study. Samples were analysed for the presence of various red cell antibodies using the Ortho glass bead, BioRad gel, Grifols gel column agglutination technologies (CAT) and Immucor Echo Solid Phase Red Cell adherence assay (SPRCA). Results from all platforms were comparable (tetrachoric correlation co-efficient > 95%). There were a few samples with low titre antibodies that could not be detected by all platforms. The results of this study demonstrate that all four platforms perform equally well, however multi centre studies would be useful to further evaluate these technologies, and to compare protocols in various laboratories. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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