Evaluation of the validity of large-scale serial sectioning TEM for three-dimensional reconstruction of rice mesophyll cells and chloroplasts.

Serial sectioning transmission electron microscopy (ssTEM) is a classical method of 3D reconstruction using serial sections obtained with an ultramicrotome. However, producing a long ribbon with homogeneity is difficult. Here, ultramicrotome movement was suspended after producing a ribbon of 15–30 s...

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Detalles Bibliográficos
Publicado en:Protoplasma Vol. 259; no. 5; pp. 1219 - 1232
Autores principales: Yamane, Koji, Oi, Takao, Taniguchi, Mitsutaka
Formato: Journal Article
Publicado: Springer Nature Sep2022
Acceso en línea:Ver este registro en EBSCOhost