Evaluation of the validity of large-scale serial sectioning TEM for three-dimensional reconstruction of rice mesophyll cells and chloroplasts.
Serial sectioning transmission electron microscopy (ssTEM) is a classical method of 3D reconstruction using serial sections obtained with an ultramicrotome. However, producing a long ribbon with homogeneity is difficult. Here, ultramicrotome movement was suspended after producing a ribbon of 15–30 s...
| Published in: | Protoplasma Vol. 259; no. 5; pp. 1219 - 1232 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Published: |
Springer Nature
Sep2022
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| Online Access: | View this record in EBSCOhost |