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Optimization of Phenolic Compounds Extraction from Wood Apple (Limonia Acidissima L.) Fruit using Experimental Design Methodology.

Wood apple is an under exploited edible fruit, that contains a myriad of phytochemicals and is used in ethnomedicine to treat a variety of diseases. This research uses Response Surface Methodology (RSM) to optimize phenolic compound extraction as significantly (p<0.01) influenced by various solvents...

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Publicado en:Current Research in Nutrition & Food Science Vol. 10; no. 2; pp. 633 - 647
Autores principales: SINGHANIA, NISHA, BARMANRAY, ARADHITA
Formato: research tables/charts Journal Article
Publicado: Current Research in Nutrition & Food Science Aug2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Aug2022
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      pub: Current Research in Nutrition & Food Science
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        10.12944/CRNFSJ.10.2.19
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        atl: Optimization of Phenolic Compounds Extraction from Wood Apple (Limonia Acidissima L.) Fruit using Experimental Design Methodology.
      aug:
        au:
          SINGHANIA, NISHA
          BARMANRAY, ARADHITA
        affil: Department of Food Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India
      sug:
        subj:
          Phenols Analysis
          Plants, Medicinal Analysis
          Fruit Analysis
          Plant Extracts Analysis
          Human
          Phytochemicals Analysis
          Temperature
          Chromatography, High Pressure Liquid
          Research Methodology
          Solvents
          Antioxidants Analysis
          India
          Free Radical Scavengers Analysis
      ab: Wood apple is an under exploited edible fruit, that contains a myriad of phytochemicals and is used in ethnomedicine to treat a variety of diseases. This research uses Response Surface Methodology (RSM) to optimize phenolic compound extraction as significantly (p<0.01) influenced by various solvents and extraction conditions including solvent concentration (50-100%), temperature (30-60oC) and time (2-5 hr) as independent variables, and extraction yield, phenolic content and DPPH activity as dependent variables. The optimal extraction conditions obtained were: methanol (81.46% concentration, 53oC temperature for 3.3 hr); ethanol (73.04% concentration, 45oC temperature for 3.58 hr); acetone (50% concentration, 43.48oC temperature for 3.06 hr) and ethyl acetate (99% concentration, 49.5oC temperature for 2 hr). The maximum extraction yield (38.14%) along with DPPH activity (80.85%) were observed using methanol, and phenolic content (248.84 mg GAE/g) using acetone. The phenolic compounds (gallic, syringic, vanillic acid etc.) in optimised solvent extract were quantified using Ultra High Performance Liquid Chromatography (UHPLC-UV).
      pubtype: Academic Journal
      doctype:
        research
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      ougenre: Article
    language: English
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