The Antitumor Effect of Topotecan Loaded Thiolated Chitosan-Dextran Nanoparticles for Intravitreal Chemotherapy: A Xenograft Retinoblastoma Model.
Purpose: This research intended to fabricate the thiolated chitosan-dextran nanoparticles (NPs) containing topotecan (TPH-CMD-TCS-NPs) to assess the ability of NPs in improving the efficacy of intravitreal chemotherapy of retinoblastoma in a rabbit xenograft model. Methods: The coacervation process...
| Publicado en: | Journal of Ophthalmic & Vision Research Vol. 18; no. 1; pp. 68 - 81 |
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| Autores principales: | , , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Knowledge E DMCC
Jan-Mar2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=162044642&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 162044642 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20082010 AY5S jtl: Journal of Ophthalmic & Vision Research issn: 20082010 maglogo: N pubinfo: dt: Jan-Mar2023 vid: 18 iid: 1 pid: 35682 pub: Knowledge E DMCC artinfo: ui: 162044642 162044642 162044642 10.18502/jovr.v18i1.12727 162044642 ppf: 68 ppct: 13 formats: fmt: @attributes: type: P tig: atl: The Antitumor Effect of Topotecan Loaded Thiolated Chitosan-Dextran Nanoparticles for Intravitreal Chemotherapy: A Xenograft Retinoblastoma Model. aug: au: Delrish, Elham Jabbarvand, Mahmoud Ghassemi, Fariba Amoli, Fahimeh Asadi Atyabi, Fatemeh Keshel, Saeed Heidari Lashay, Alireza Mirzazadeh Tekie, Farnaz Sadat Soleimani, Masoud Dinarvand, Rassoul affil: Translational Ophthalmology Research Centre (TORC), Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran sug: subj: Retinoblastoma Drug Therapy Topotecan Therapeutic Use Polysaccharides Administration and Dosage Dextrans Administration and Dosage Nanoparticle Drug Delivery System Chemotherapy, Cancer Xenografts Animal Studies Rabbits Topotecan Administration and Dosage Microscopy Methods Eye Drug Effects Dyes Flow Cytometry Injections Necrosis Staining and Labeling Methods Tumor Burden Calorimetry Time Factors Outcome Assessment Kruskal-Wallis Test Data Analysis Software Post Hoc Analysis Descriptive Statistics Funding Source ab: Purpose: This research intended to fabricate the thiolated chitosan-dextran nanoparticles (NPs) containing topotecan (TPH-CMD-TCS-NPs) to assess the ability of NPs in improving the efficacy of intravitreal chemotherapy of retinoblastoma in a rabbit xenograft model. Methods: The coacervation process was used to produce the NPs. The cellular uptake of Cyanine-3 (CY3)-labeled NPs were investigated in human retinoblastoma Y79 cells using confocal microscopy. Also, the prepared TPH-CMD-TCS-NPs were tested in vitro by the tetrazolium dyes II (XTT) and flow cytometry in order to assess their cytotoxicity. In addition, a rabbit xenograft model of retinoblastoma was developed to test the antitumor effectiveness of TPH-CMD-TCS-NPs through intravitreal administration. Results: NPs had a mean diameter, polydispersity index, and zeta potential of 30 ± 4 nm, 0.24 ± 0.03 and +10 ± 3 mV, respectively. NPs (IC50s 40.40 compared to 126.20 nM, P = 0.022) were more effective than free topotecan as a dose-based feature. The tumor reaction to intravitreal chemotherapy with NPs was measured by evaluating the percentage of necrosis in the tumor tissue (91 ± 2%) and vitreous seeds (89 ± 9%) through hematoxylin and eosin (H&E) staining. In comparison with the control group, the TPH-CMD-TCs-NPs treated group showed a significant decrease in tumor volume seven days after the intravitreal injection (P = 0.039). No significant changes were found in the ERG parameters after the intravitreal injection of TPH-CMD-TCs-NPs or TPH (P > 0.05). Conclusion: This investigation revealed definitive antitumor efficacy of TPH-CMD-TCS-NPs by intravitreal administration in the rabbit xenograft retinoblastoma model. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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