Rapid point of care testing for four bacterial sexually transmitted infections using the portable isothermal loop-mediated nucleic acid amplification eazyplex platform.
Purpose: To analyze sensitivity and specificity of the rapid point-of-care (POC) eazyplex testing platform for bacterial sexually transmitted infections (STI) among men who have sex with men (MSM). Methods: 272 anal, urethral, and pharyngeal swabs collected from 153 MSM were tested by both the eazyp...
| Publicado en: | Infection Vol. 51; no. 4; pp. 1141 - 1146 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Aug2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=164982773&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 164982773 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Aug2023 vid: 51 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 164982773 161380141 164982773 164982773 10.1007/s15010-023-01981-5 164982773 ppf: 1141 ppct: 5 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Rapid point of care testing for four bacterial sexually transmitted infections using the portable isothermal loop-mediated nucleic acid amplification eazyplex platform. aug: au: Esitgen Germaner, Ece T. Wassill, Lars Dichtl, Karl Roider, Julia Seybold, Ulrich affil: Sektion Klinische Infektiologie, Med. Klinik und Poliklinik IV, LMU Klinikum Innenstadt, Ludwig-Maximilians-Universität München, Pettenkoferstr. 8a, 80336, Munich, Germany sug: subj: Rapid On-Site Evaluation Sexually Transmitted Diseases, Bacterial Diagnosis Loop-Mediated Isothermal Amplification Methods Sensitivity and Specificity Men Who Have Sex With Men Human Male Anus Microbiology Urethra Microbiology Pharynx Microbiology Academic Medical Centers Comparative Studies Polymerase Chain Reaction Gonorrhea Descriptive Statistics Male ab: Purpose: To analyze sensitivity and specificity of the rapid point-of-care (POC) eazyplex testing platform for bacterial sexually transmitted infections (STI) among men who have sex with men (MSM). Methods: 272 anal, urethral, and pharyngeal swabs collected from 153 MSM were tested by both the eazyplex platform and an in-house PCR or culture in the university microbiology reference laboratory. Results: Compared to the reference diagnostic method, the overall sensitivity/specificity of eazyplex was 88%/98% for N. gonorrhoeae, 82%/100% for C. trachomatis, 70%/ > 99% for U. urealyticum, and 85%/98% for M. hominis, respectively. Sensitivity for N. gonorrhoeae and U. urealyticum in urethral samples was 100%. Conclusion: With good to very good sensitivity depending on the sampling site and pathogen as well as very good specificity overall the eazyplex platform is a useful rapid diagnostic method for POC bacterial STI-testing especially for N. gonorrhoeae and C. trachomatis, allowing for almost immediate treatment initiation. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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