Metabolic signature of 13C-labeled wheat bran consumption related to gut fermentation in humans: a pilot study.
Purpose: The aim of this pilot study was to analyze concomitantly the kinetics of production of 13C-labeled gut-derived metabolites from 13C-labeled wheat bran in three biological matrices (breath, plasma, stools), in order to assess differential fermentation profiles among subjects. Methods: Six he...
| Publicado en: | European Journal of Nutrition Vol. 62; no. 6; pp. 2633 - 2649 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Sep2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=169912267&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 169912267 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Sep2023 vid: 62 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 169912267 163883753 169912267 169912267 10.1007/s00394-023-03161-5 169912267 ppf: 2633 ppct: 16 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Metabolic signature of 13C-labeled wheat bran consumption related to gut fermentation in humans: a pilot study. aug: au: Meiller, Laure Sauvinet, Valérie Breyton, Anne-Esther Ranaivo, Harimalala Machon, Christelle Mialon, Anne Meynier, Alexandra Bischoff, Stephan C. Walter, Jens Neyrinck, Audrey M. Laville, Martine Delzenne, Nathalie M. Vinoy, Sophie Nazare, Julie-Anne affil: Centre de Recherche en Nutrition Humaine Rhône-Alpes, Univ-Lyon, INSERM, INRAe, Claude Bernard Lyon1 University, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, Pierre Bénite, France sug: subj: Dietary Fiber Metabolism Wheat Metabolism Fermentation Gut Microbiota Metabolism Breakfast Metabolites Kinetics Fatty Acids, Volatile Human Pilot Studies Female Adult Gas Chromatography-Mass Spectrometry Butyric Acids Gene Expression RNA Adult: 19-44 years Female ab: Purpose: The aim of this pilot study was to analyze concomitantly the kinetics of production of 13C-labeled gut-derived metabolites from 13C-labeled wheat bran in three biological matrices (breath, plasma, stools), in order to assess differential fermentation profiles among subjects. Methods: Six healthy women consumed a controlled breakfast containing 13C-labeled wheat bran biscuits. H2, CH4 and 13CO2, 13CH4 24 h-concentrations in breath were measured, respectively, by gas chromatography (GC) and GC-isotope ratio mass spectrometry (GC-IRMS). Plasma and fecal concentrations of 13C-short-chain fatty acids (linear SCFAs: acetate, propionate, butyrate, valerate; branched SCFAs: isobutyrate, isovalerate) were quantified using GC-combustion-IRMS. Gut microbiota composition was assessed by16S rRNA gene sequencing analysis. Results: H2 and CH4 24 h-kinetics distinguished two groups in terms of fermentation-related gas excretion: high-CH4 producers vs low-CH4 producers (fasting concentrations: 45.3 ± 13.6 ppm vs 6.5 ± 3.6 ppm). Expired 13CH4 was enhanced and prolonged in high-CH4 producers compared to low-CH4 producers. The proportion of plasma and stool 13C-butyrate tended to be higher in low-CH4 producers, and inversely for 13C-acetate. Plasma branched SCFAs revealed different kinetics of apparition compared to linear SCFAs. Conclusion: This pilot study allowed to consider novel procedures for the development of biomarkers revealing dietary fiber-gut microbiota interactions. The non-invasive assessment of exhaled gas following 13C-labeled fibers ingestion enabled to decipher distinct fermentation profiles: high-CH4 producers vs low-CH4 producers. The isotope labeling permits a specific in vivo characterisation of the dietary fiber impact consumption on microbiota metabolite production. Clinical trial registration: The study has been registered under the number NCT03717311 at ClinicalTrials.gov on October 24, 2018. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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