Structural modifications toward improved lead-203/lead-212 peptide-based image-guided alpha-particle radiopharmaceutical therapies for neuroendocrine tumors.

Purpose: The lead-203 (203Pb)/lead-212 (212Pb) elementally identical radionuclide pair has gained significant interest in the field of image-guided targeted alpha-particle therapy for cancer. Emerging evidence suggests that 212Pb-labeled peptide-based radiopharmaceuticals targeting somatostatin rece...

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Publicado en:European Journal of Nuclear Medicine & Molecular Imaging Vol. 51; no. 4; pp. 1147 - 1163
Autores principales: Lee, Dongyoul, Li, Mengshi, Liu, Dijie, Baumhover, Nicholas J., Sagastume, Edwin A., Marks, Brenna M., Rastogi, Prerna, Pigge, F. Christopher, Menda, Yusuf, Johnson, Frances L., Schultz, Michael K.
Formato: Journal Article
Publicado: Springer Nature Mar2024
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Mar2024
      vid: 51
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      pub: Springer Nature
      place: New York, New York
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        175566835
        173583550
        10.1007/s00259-023-06494-9
        175566835
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        atl: Structural modifications toward improved lead-203/lead-212 peptide-based image-guided alpha-particle radiopharmaceutical therapies for neuroendocrine tumors.
      aug:
        au:
          Lee, Dongyoul
          Li, Mengshi
          Liu, Dijie
          Baumhover, Nicholas J.
          Sagastume, Edwin A.
          Marks, Brenna M.
          Rastogi, Prerna
          Pigge, F. Christopher
          Menda, Yusuf
          Johnson, Frances L.
          Schultz, Michael K.
        affil: https://ror.org/024ctqw02 Department of Physics and Chemistry, Korea Military Academy, Seoul, Republic of Korea
      sug:
      ab: Purpose: The lead-203 (203Pb)/lead-212 (212Pb) elementally identical radionuclide pair has gained significant interest in the field of image-guided targeted alpha-particle therapy for cancer. Emerging evidence suggests that 212Pb-labeled peptide-based radiopharmaceuticals targeting somatostatin receptor subtype 2 (SSTR2) may provide improved effectiveness compared to beta-particle-based therapies for neuroendocrine tumors (NETs). This study aims to improve the performance of SSTR2-targeted radionuclide imaging and therapy through structural modifications to Tyr3-octreotide (TOC)-based radiopharmaceuticals. Methods: New SSTR2-targeted peptides were designed and synthesized with the goal of optimizing the incorporation of Pb isotopes through the use of a modified cyclization technique; the introduction of a Pb-specific chelator (PSC); and the insertion of polyethylene glycol (PEG) linkers. The binding affinity of the peptides and the cellular uptake of 203Pb-labeled peptides were evaluated using pancreatic AR42J (SSTR2+) tumor cells and the biodistribution and imaging of the 203Pb-labeled peptides were assessed in an AR42J tumor xenograft mouse model. A lead peptide was identified (i.e., PSC-PEG2-TOC), which was then further evaluated for efficacy in 212Pb therapy studies. Results: The lead radiopeptide drug conjugate (RPDC) — [203Pb]Pb-PSC-PEG2-TOC — significantly improved the tumor-targeting properties, including receptor binding and tumor accumulation and retention as compared to [203Pb]Pb-DOTA0-Tyr3-octreotide (DOTATOC). Additionally, the modified RPDC exhibited faster renal clearance than the DOTATOC counterpart. These advantageous characteristics of [212Pb]Pb-PSC-PEG2-TOC resulted in a dose-dependent therapeutic effect with minimal signs of toxicity in the AR42J xenograft model. Fractionated administrations of 3.7 MBq [212Pb]Pb-PSC-PEG2-TOC over three doses further improved anti-tumor effectiveness, resulting in 80% survival (70% complete response) over 120 days in the mouse model. Conclusion: Structural modifications to chelator and linker compositions improved tumor targeting and pharmacokinetics (PK) of 203/212Pb peptide-based radiopharmaceuticals for NET theranostics. These findings suggest that PSC-PEG2-TOC is a promising candidate for Pb-based targeted radionuclide therapy for NETs and other types of cancers that express SSTR2.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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