Polymerase Evolution: Efforts toward Expansion of the Genetic Code.

This article focuses on a study related to polymerase evolution. Expansion of the alphabet to contain a third base pair would allow additional information to be encoded in DNA and would also enable a variety of in vitro experiments using unnatural nucleic acids. One strategy for improving the replic...

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Detalles Bibliográficos
Publicado en:	Journal of the American Chemical Society Vol. 127; no. 36; pp. 12470 - 12472
Autores principales:	Leconte, Aaron M., Chen, Liangjing, Romesberg, Floyd E.
Formato:	Artículo
Publicado:	American Chemical Society 9/14/2005
Materias:	Nucleic acids DNA Polymerase chain reaction Biomolecules DNA replication Research
Acceso en línea:	Ver este registro en EBSCOhost

Descripción
Sumario:	This article focuses on a study related to polymerase evolution. Expansion of the alphabet to contain a third base pair would allow additional information to be encoded in DNA and would also enable a variety of in vitro experiments using unnatural nucleic acids. One strategy for improving the replication of these unnatural base pairs is based on their further derivatization with heteroatoms or alkyl substituents. This strategy mimics the optimization of the natural bases in nature. To optimize the replication of DNA containing PICS, researchers decided to select for variants of Sf that more efficiently synthesize and extend a primer-template terminus containing the self-pair.