Polymerase Evolution: Efforts toward Expansion of the Genetic Code.

This article focuses on a study related to polymerase evolution. Expansion of the alphabet to contain a third base pair would allow additional information to be encoded in DNA and would also enable a variety of in vitro experiments using unnatural nucleic acids. One strategy for improving the replic...

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Publicado en:Journal of the American Chemical Society Vol. 127; no. 36; pp. 12470 - 12472
Autores principales: Leconte, Aaron M., Chen, Liangjing, Romesberg, Floyd E.
Formato: Artículo
Publicado: American Chemical Society 9/14/2005
Materias:
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 9/14/2005
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      pub: American Chemical Society
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        atl: Polymerase Evolution: Efforts toward Expansion of the Genetic Code.
      aug:
        au:
          Leconte, Aaron M.
          Chen, Liangjing
          Romesberg, Floyd E.
        affil: Department of Chemistry, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037.
      su:
        Nucleic acids
        DNA
        Polymerase chain reaction
        Biomolecules
        DNA replication
        Research
      sug:
        subj:
          Nucleic acids
          DNA
          Polymerase chain reaction
          Biomolecules
          DNA replication
          Research
      ab: This article focuses on a study related to polymerase evolution. Expansion of the alphabet to contain a third base pair would allow additional information to be encoded in DNA and would also enable a variety of in vitro experiments using unnatural nucleic acids. One strategy for improving the replication of these unnatural base pairs is based on their further derivatization with heteroatoms or alkyl substituents. This strategy mimics the optimization of the natural bases in nature. To optimize the replication of DNA containing PICS, researchers decided to select for variants of Sf that more efficiently synthesize and extend a primer-template terminus containing the self-pair.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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