Suppression of TRIM72‐mediated endoplasmic reticulum stress facilitates FOXM1 promotion of diabetic ulcer healing.
Foot ulcers are amongst the most prevalent complications of diabetes, known for their delayed healing process. Recent research indicates that the transcription factor forkhead box M1 (FOXM1) plays a role in promoting diabetic ulcer repair. However, the precise mechanisms underlying FOXM1 functions i...
| Publicado en: | Wound Repair & Regeneration Vol. 33; no. 1; pp. 1 - 14 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jan/Feb2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=183688081&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 183688081 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jan/Feb2025 vid: 33 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 183688081 183688081 183688081 10.1111/wrr.13247 183688081 ppf: 1 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Suppression of TRIM72‐mediated endoplasmic reticulum stress facilitates FOXM1 promotion of diabetic ulcer healing. aug: au: Peng, Lingling Tian, Yaning Wu, Xiangkai Liu, Fengqi Zhou, Mingzhu Wu, Zixi Xia, Yumin Liu, Xiaoming Cheng, Chuantao affil: Department of Dermatology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China sug: subj: Transcription Factors Physiology DNA-Binding Proteins Physiology Carrier Proteins Physiology Endoplasmic Reticulum Stress Diabetic Foot Wound Healing Cell Physiology Human Animal Studies Mice Immunohistochemistry Gene Expression Cell Line In Vitro Studies Cell Proliferation Cell Movement Biological Assay Funding Source ab: Foot ulcers are amongst the most prevalent complications of diabetes, known for their delayed healing process. Recent research indicates that the transcription factor forkhead box M1 (FOXM1) plays a role in promoting diabetic ulcer repair. However, the precise mechanisms underlying FOXM1 functions in this context remain unclear. This study aimed to clarify the role of tripartite motif‐containing protein 72 (TRIM72)‐mediated endoplasmic reticulum stress in FOXM1 promotive effects. Immunohistochemistry revealed that FOXM1 expression was significantly reduced in the lesion tissues of diabetic foot ulcer patients. In vitro experiments revealed a decrease in FOXM1 expression in cultured dermal fibroblasts under high glucose conditions. Activating FOXM1 with a plasmid accelerated the proliferation, migration, and differentiation of dermal fibroblasts and mitigated endoplasmic reticulum stress under high glucose conditions. Additionally, ChIP and luciferase reporter gene assays confirmed that FOXM1 suppressed TRIM72 expression transcriptionally by binding to its promoter. Furthermore, high glucose induced ubiquitination of adenosine 5′‐monophosphate‐activated protein kinase alpha (AMPKα), whilst inactivation of AMPKα signalling reversed the aforementioned effects of FOXM1 on cells. Finally, the FOXM1‐overexpressing plasmid was transfected in vivo, which promoted wound healing in a murine diabetic ulcer model. In conclusion, FOXM1 reduces endoplasmic reticulum stress by inhibiting TRIM72‐mediated AMPKα ubiquitination, thereby accelerating the healing of diabetic ulcers. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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