Anti-diabetic and anti-urease inhibition potential of Amomum dealbatum Roxb. seeds through a bioassay-guided approach.

Using HPLC-PDA and HRMS analysis, five compounds p-coumaric acid, sinapic acid, quercetin, trans-ferulic and gallic acid were identified in seeds of Amomum dealbatum Roxb. The GC-MS analysis identified 1-dodecanol, phenol, 3,5-bis(1,1-dimethylethyl), Oleic Acid and 1-Heptacosanol which possess anti-...

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Publicado en:Natural Product Research Vol. 39; no. 10; pp. 2978 - 2984
Autores principales: Sonia, Hage, Chelleng, Nilamoni, Afzal, Nazim Uddin, Manna, Prasenjit, Puzari, Minakshi, Chetia, Pankaj, Tamuly, Chandan
Formato: Journal Article
Publicado: Taylor & Francis Ltd May2025
Acceso en línea:Ver este registro en EBSCOhost
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      dt: May2025
      vid: 39
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      pub: Taylor & Francis Ltd
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        185229113
        174681405
        10.1080/14786419.2023.2301679
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        atl: Anti-diabetic and anti-urease inhibition potential of Amomum dealbatum Roxb. seeds through a bioassay-guided approach.
      aug:
        au:
          Sonia, Hage
          Chelleng, Nilamoni
          Afzal, Nazim Uddin
          Manna, Prasenjit
          Puzari, Minakshi
          Chetia, Pankaj
          Tamuly, Chandan
        affil: CSIR-North East Institute of Science and Technology, Arunachal, India
      sug:
      ab: Using HPLC-PDA and HRMS analysis, five compounds p-coumaric acid, sinapic acid, quercetin, trans-ferulic and gallic acid were identified in seeds of Amomum dealbatum Roxb. The GC-MS analysis identified 1-dodecanol, phenol, 3,5-bis(1,1-dimethylethyl), Oleic Acid and 1-Heptacosanol which possess anti-diabetic properpties. A bioassay-guided technique was used to determine the degree of inhibition that A. dealbatum seeds crude methanol extract and its most active sub-fraction had against the α-glucosidase and Helicobacter pylori urease enzymes. In the Rat L6 myoblast cell line, glucose absorption through the GLUT4 transporter of most active subfraction (EASF80) was examined. According to a molecular docking investigation, these compounds strongly interacted with the GLUT4 transporter, H pylori and α-glucosidase enzyme. Sinapic acid interacted most strongly with the H. pylori urease enzyme while gallic acid interacted with both the α-glucosidase enzyme and the GLUT4 transporter. Additionally, a molecular docking simulation study was carried out to recognise the stability of the complexes.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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