| Sumario: | Due to their wide range of applications in modern technology, silver metallic nanomaterials have garnered considerable attention, driving significant research and development efforts. This study focuses on investigating the antimicrobial properties of silver nanoparticles (Ag‐NPs), encompassing their production, characterization, and biological aspects. The eco‐friendly extracellular biosynthetic method employed in this work utilizes extracts from the marine fungus Aspergillus flavus MK4 as reducing agents for nanoparticle synthesis. The analysis of colloidal Ag‐NPs using ultraviolet‐visible spectroscopy revealed a peak in absorbance at 460 nm, indicating the presence of plasmonic properties. Electron imaging of the internal structure (TEM) elucidated the spherical shape of Ag‐NPs, which measure 15 nm in size. Testing against bacterial diversities revealed potent antibacterial and antifungal efficacy. Cytotoxicity against the HepG‐2 cell line was assessed using the MTT assay, and antioxidant properties were examined through the radical scavenging (DPPH) assay. Based on their conceivable applications in the fields of antibacterial, anticancer, and wound healing, the synthesized Ag‐NPs exhibit promising features. With its powerful ability to synthesize Ag‐NPs, A. flavus MK4 can generate extensively characterized nanoparticles, demonstrating their antibacterial, antioxidant, anticancer, and wound‐healing properties.
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