| Sumario: | The pain of the blood sampling process is an unpleasant and common experience in the treatment process. Stimulation the thalamus structure and corticothalamic pathways with at specific frequencies could play a role in modulating pain perception. The aim of this study was to investigate the use low acoustic frequencies in reducing pain during arterial and venous blood sampling. In a triple-blinded clinical trial study, 300 patients in each process were randomly divided into control and intervention groups. Patients in the intervention groups received sound frequencies of 10–200 Hz with an intensity of 50 dB during the procedures. Pain intensity, heart rate changes, number of attempts for successful sampling, and time required for each procedure were measured. Data were analyzed using Prism 10 software. Pain levels were significantly lower in the intervention groups with increasing sound frequencies in both procedures (P < 0.05). The duration of blood sampling procedures was significantly shorter with increasing sound frequency (P < 0.05), but no difference was observed in the frequency of sampling attempts (P > 0.05). By increasing the sound frequency, the intensity of tachycardia significantly decreased following the blood sampling process (P < 0.05), especially at higher frequencies. Applying low sound frequencies effectively could reduce acute pain and its consequences resulting from an invasive process. Sound frequencies appear to play a role in modulating pain pathways in the central nervous system. The study findings suggest that sound frequency ranges as a non-pharmacological analgesia intervention can be used to reduce pain and discomfort following a variety of blood sampling procedures, which performed by nurses.
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