| Sumario: | The detailed chemical investigation of the South China Sea soft coral Pachyclavularia violacea, led to the isolation and characterization of a new casbane-type diterpenoid, 7,8-epoxy-casbene (1), along with seven known compounds (2–8). Their structures were determined by extensive spectroscopic analysis, quantum mechanical nuclear magnetic resonance (QM-NMR) method, time-dependent density functional theory electronic circular dichroism (TDDFT-ECD) calculation, and comparison with the spectral data previously reported in the literature. In in vitro bioassays, the anti-inflammatory activities of the isolated compounds were evaluated in the lipopolysaccharide (LPS)-induced RAW 264.7 cells, and compounds 6–8 displayed interesting inhibitory effects on LPS-induced tumor necrosis factor (TNF)-α protein release with half-maximal inhibitory concentration (IC50) values of 201.1, 231.7, and 66.21 µM, respectively.
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