| Summary: | Investigation of the Miliusa sessilis hexane extract led to four novel lanostane triterpenoids, miliseol A–D (1–4) and five known compounds (5–9). Through extensive spectroscopic analyses, the new compounds were identified as (3β,23S)-23-methoxy-24-methylene-29-nor-5α-lanost-9(11)-en-3-ol (1), (3β,23S)-23-methoxy-24-methylene-5α-lanost-9(11)-en-3-ol (2), (3β,16β)-24-methylene-5α-lanost-9(11)-ene-3,16-diol (3), and (3β,24S)-24,241-epoxy-5α-lanost-9(11)-en-3-ol (4). The density functional theory (DFT) computations combined with a statistical procedure (DP4+) were used to identify the stereochemistry of compound 4. The X-ray crystallographic data was utilised to confirm the absolute configurations of compounds 1 and 3. The known compounds were isolated and elucidated as (+)-spathulenol (5), phytol (6), T-muurolol (7), β-sitosterol (8), and β-sitosterol-3-O-β-d-glucopyranoside (9) through spectroscopic analyses and comparison with the literature. Biological activity screening indicated that compound 1 promoted cell migration in the HaCaT cell line, with minimal cytotoxicity at the tested concentrations. This finding suggests its potential as an enhancing agent for skin wound healing.
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