Comparative evaluation of osteoblastic cell proliferation and cell adhesion on injectable platelet-rich fibrin-coated versus uncoated airborne-particle abraded, large grit, and acid-etched titanium implants: An in vitro study.
Despite the increasing use of injectable platelet rich fibrin (i-PRF) in dentistry for its regenerative potential, a significant lack of evidence exists regarding its impact on early cellular responses, particularly osteoblastic cell proliferation and cell adhesion on airborne-particle abraded, larg...
| Publicado en: | Journal of Prosthetic Dentistry Vol. 136; no. 3; pp. 821 - 829 |
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| Autores principales: | , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Sep2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| Sumario: | Despite the increasing use of injectable platelet rich fibrin (i-PRF) in dentistry for its regenerative potential, a significant lack of evidence exists regarding its impact on early cellular responses, particularly osteoblastic cell proliferation and cell adhesion on airborne-particle abraded, large grit and acid etched titanium (SLA-Ti) implant surfaces. The purpose of this study was to evaluate and compare the initial osteoblastic cell proliferation and cell adhesion on uncoated and i-PRF coated SLA titanium implant surfaces. Eleven commercially available SLA-Ti implants (4.2×16-mm) were tested. Each implant was sectioned into 4 4.2×4-mm specimens and sterilized. A total of 42 specimens were then randomly divided into 2 groups (n =21) of uncoated and 20% i-PRF coated implants. Each group was further subdivided into 2 subgroups for evaluation of osteoblastic cell proliferation (n =10) and cell adhesion (n =11). MG-63 osteoblast-like cells were cultured and seeded onto each implant specimen. Cell proliferation was evaluated using the methyl thiazolyl tetrazolium assay after 72 hours with optical density values measured at 540 nm. Cell adhesion count was evaluated using laser scanning confocal microscopy after 72 hours of cell culturing and quantified using an automated cell counting tool in the Image J software program. Data obtained were statistically analyzed using the Kruskal-Wallis and the independent Student t test for cell proliferation and cell adhesion respectively (ɑ=.05). The i-PRF coated implant specimens showed significantly higher osteoblastic cell proliferation (0.73) and cell adhesion (5390 ±643) as compared to osteoblastic cell proliferation (0.71) and cell adhesion (2612 ±709) in uncoated implant specimens (P <.001). i-PRF coated SLA-Ti implant specimens demonstrated elevated osteoblastic cell proliferation and cell adhesion as compared to uncoated specimens which may promote early healing, bone formation and accelerate osseointegration. |
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