Evidence for Two Ferryl Species in Chioroperoxidase Compound II.
Using a combination of density functional calculations and Mössbauer spectroscopy, we have examined chloroperoxidase compound II (CPO-II). The Mössbauer spectrum of CPO-ll suggests the presence of two distinct ferryl species in an ~70:30 ratio. Density functional calculations and cryogenic reduction...
| Publicado en: | Journal of the American Chemical Society Vol. 128; no. 18; pp. 6147 - 6154 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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American Chemical Society
5/10/2006
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=21109617&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 21109617 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 5/10/2006 vid: 128 iid: 18 pid: 997 pub: American Chemical Society artinfo: ui: 21109617 10.1021/ja057876w ppf: 6147 ppct: 7 formats: tig: atl: Evidence for Two Ferryl Species in Chioroperoxidase Compound II. aug: au: Stone, Karl L. Hoffart, Lee M. Behan, Rachel K. Krebs, Carsten Green, Michael T. affil: Contribution from the Department of Chemistry and Biochemistry, The Pennsylvania State University, University Park, Pennsylvania 16802 Department of Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802 su: Peroxidase Iron compounds Mössbauer spectroscopy Chlorine compounds Density functionals Hydrogen-ion concentration sug: subj: Peroxidase Iron compounds Mössbauer spectroscopy Chlorine compounds Density functionals Hydrogen-ion concentration ab: Using a combination of density functional calculations and Mössbauer spectroscopy, we have examined chloroperoxidase compound II (CPO-II). The Mössbauer spectrum of CPO-ll suggests the presence of two distinct ferryl species in an ~70:30 ratio. Density functional calculations and cryogenic reduction and annealing experiments allow us to assign the major species as an Fe(IV)OH intermediate. The Mössbauer parameters of the minor component are indicative of an authentic iron(IV)oxo species, but we have found the 70:30 ratio to be pH invariant. The unchanging ratio of component concentrations is in agreement with CPO-ll's visible absorption spectrum, which shows no change over the enzyme's range of pH stability. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2006 holdings: @attributes: islocal: N |
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