Cell-Permeable Peptide Nucleic Acid Designed to Bind to the 5′-Untranslated Region of E-cadherin Transcript Induces Potent and Sequence-Specific Antisense Effects.

Establishing a general and effective method for regulating gene expression in mammalian systems is important for many aspects of biological and biomedical research. Herein we report the antisense activities of a cell-permeable, guanidine-based peptide nucleic acid (PNA) called GPNA. We show that a G...

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Detalles Bibliográficos
Publicado en:	Journal of the American Chemical Society Vol. 128; no. 50; pp. 16104 - 16113
Autores principales:	Dragulescu-Andrasi, Anca, Rapireddy, Srinivas, He, Gaofei, Bhattacharya, Birendra, Hyldig-Nielsen, Jens J., Zon, Gerald, Ly, Danith H.
Formato:	Artículo
Publicado:	American Chemical Society 12/20/2006
Materias:	Gene expression Mammal cytology Peptides Proteins Antisense nucleic acids Guanidine Oligomers Biochemical research
Acceso en línea:	Ver este registro en EBSCOhost

Descripción
Sumario:	Establishing a general and effective method for regulating gene expression in mammalian systems is important for many aspects of biological and biomedical research. Herein we report the antisense activities of a cell-permeable, guanidine-based peptide nucleic acid (PNA) called GPNA. We show that a GPNA oligomer designed to bind to the transcriptional start-site of human E-cadherin gene induces potent and sequence-specific antisense effects and is less toxic to the cells than the corresponding PNA-polyarginine conjugate. GPNA confers its silencing effect by blocking protein translation. The findings reported in this study provide a molecular framework for designing the next generation cell-permeable nucleic acid mimics for regulating gene expression in live cells and intact organisms.