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Subdiffraction Imaging through the Selective Donut-Mode Depletion of Thermally Stable Photoswitchable Fluorophores: Numerical Analysis and Application to the Fluorescent Protein Dronpa.

The fast and reversible on/off switching of the fluorescence emission of the GFP-like fluorescent protein Dronpa has attracted considerable interest for applications in subdiffraction imaging. In this paper we study the use of a donut-mode beam in combination with two more overlapping laser beams to...

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Published in:Journal of the American Chemical Society Vol. 129; no. 51; pp. 16132 - 16142
Main Authors: Dedecker, Peter, Hotta, Jun-ichi, FIors, Cristina, Sliwa, Michel, Uji-i, Hiroshi, Roeffaers, Maarten B. J., Ando, Ryoko, Mizuno, Hideaki, Miyawaki, Atsushi, Hofkens, Johan
Format: Article
Published: American Chemical Society 12/26/2007
Subjects:
Numerical analysis
Green fluorescent protein
Fluorescence
Laser beams
Optical resolution
Optical diffraction
Online Access:View this record in EBSCOhost