Discovery, Characterization, and Optimization of an Unnatural Base Pair for Expansion of the Genetic Alphabet.
DNA is inherently limited by its four natural nucleotides. Efforts to expand the genetic alphabet, by addition of an unnatural base pair, promise to expand the biotechnological applications available for DNA as well as to be an essential first step toward expansion of the genetic code. We have condu...
| Publicado en: | Journal of the American Chemical Society Vol. 130; no. 7; pp. 2336 - 2344 |
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| Autores principales: | , , , , , |
| Formato: | Artículo |
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American Chemical Society
2/20/2008
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=31215077&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 31215077 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 2/20/2008 vid: 130 iid: 7 pid: 997 pub: American Chemical Society artinfo: ui: 31215077 10.1021/ja078223d ppf: 2336 ppct: 8 formats: tig: atl: Discovery, Characterization, and Optimization of an Unnatural Base Pair for Expansion of the Genetic Alphabet. aug: au: Leconte, Aaron M. Gil Tae Hwang Matsuda, Shigeo Capek, Petr Han, Yoshiyuki Romesberg, Floyd E. affil: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California, 92037 su: Genes Genetic code Nucleotide analysis DNA polymerases Nucleotide sequence Biotechnology sug: subj: Genes Genetic code Nucleotide analysis DNA polymerases Nucleotide sequence Biotechnology ab: DNA is inherently limited by its four natural nucleotides. Efforts to expand the genetic alphabet, by addition of an unnatural base pair, promise to expand the biotechnological applications available for DNA as well as to be an essential first step toward expansion of the genetic code. We have conducted two independent screens of hydrophobic unnatural nucleotides to identify novel candidate base pairs that are well recognized by a natural DNA polymerase. From a pool of 3600 candidate base pairs, both screens identified the same base pair, dSICS:dMMO2, which we report here. Using a series of related analogues, we performed a detailed structure-activity relationship analysis, which allowed us to identify the essential functional groups on each nucleobase. From the results of these studies, we designed an optimized base pair, d5SICS:dMMO2, which is efficiently and selectively synthesized by Kf within the context of natural DNA. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2008 holdings: @attributes: islocal: N |
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