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Superresolution Microscopy on the Basis of Engineered Dark States.

The article focuses on the development in the concept of fluorescence microscopy which includes stimulated emission depletion (STED) microscopy and sub diffraction resolution imaging. Sub diffraction resolution microscopy is localized by imaging with a sensitive camera that rely on photoactivatable...

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Published in:Journal of the American Chemical Society Vol. 130; no. 50; pp. 16840 - 16842
Main Authors: Steinhauer, Christian, Forthmann, Carsten, Vogelsang, Jan, Tinnefeld, Philip
Format: Article
Published: American Chemical Society 12/17/2008
Subjects:
Fluorescence microscopy
Imaging systems
Optical diffraction
Photographic optics
Optics
Online Access:View this record in EBSCOhost
Summary:The article focuses on the development in the concept of fluorescence microscopy which includes stimulated emission depletion (STED) microscopy and sub diffraction resolution imaging. Sub diffraction resolution microscopy is localized by imaging with a sensitive camera that rely on photoactivatable or photoswitchable fluorophores. Engineering dark states of desired duration are allowed by controlling the photophysics.