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A Ratiometric pH Reporter For Imaging Protein-dye Conjugates In Living Cells.

The article presents a study on ratiometric pH probes for intracellular imaging. It describes the pH probe 1 which is based on the thorough-bond energy transfer cassette, and the demonstration which indicates that probe 1 tends to fluoresce with higher quantum yields than C.SNARF-1 over a physiologi...

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Publicado en:Journal of the American Chemical Society Vol. 131; no. 5; pp. 1642 - 1644
Autores principales: Han, Junyan, Loudet, Aurore, Barhoumi, Rola, Burghardt, Robert C., Burgess, Kevin
Formato: Artículo
Publicado: American Chemical Society 2/11/2009
Materias:
Hydrogen-ion concentration
Imaging systems
Energy transfer
Molecular probes
Fluorescent probes
Green fluorescent protein
Fluorescent polymers
Acceso en línea:Ver este registro en EBSCOhost
Sumario:The article presents a study on ratiometric pH probes for intracellular imaging. It describes the pH probe 1 which is based on the thorough-bond energy transfer cassette, and the demonstration which indicates that probe 1 tends to fluoresce with higher quantum yields than C.SNARF-1 over a physiologically relevant pH range. It notes that the energy transfer approach to pH probes for intracellular imaging has considerable potential for modifications with other pH sensitive donors.