A Ratiometric pH Reporter For Imaging Protein-dye Conjugates In Living Cells.

The article presents a study on ratiometric pH probes for intracellular imaging. It describes the pH probe 1 which is based on the thorough-bond energy transfer cassette, and the demonstration which indicates that probe 1 tends to fluoresce with higher quantum yields than C.SNARF-1 over a physiologi...

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Publicado en:Journal of the American Chemical Society Vol. 131; no. 5; pp. 1642 - 1644
Autores principales: Han, Junyan, Loudet, Aurore, Barhoumi, Rola, Burghardt, Robert C., Burgess, Kevin
Formato: Artículo
Publicado: American Chemical Society 2/11/2009
Materias:
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2/11/2009
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      pub: American Chemical Society
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        atl: A Ratiometric pH Reporter For Imaging Protein-dye Conjugates In Living Cells.
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        au:
          Han, Junyan
          Loudet, Aurore
          Barhoumi, Rola
          Burghardt, Robert C.
          Burgess, Kevin
        affil:
          Department of Chemistry
          Department of Veterinary Integrative Bioscience
      su:
        Hydrogen-ion concentration
        Imaging systems
        Energy transfer
        Molecular probes
        Fluorescent probes
        Green fluorescent protein
        Fluorescent polymers
      sug:
        subj:
          Hydrogen-ion concentration
          Imaging systems
          Energy transfer
          Molecular probes
          Fluorescent probes
          Green fluorescent protein
          Fluorescent polymers
      ab: The article presents a study on ratiometric pH probes for intracellular imaging. It describes the pH probe 1 which is based on the thorough-bond energy transfer cassette, and the demonstration which indicates that probe 1 tends to fluoresce with higher quantum yields than C.SNARF-1 over a physiologically relevant pH range. It notes that the energy transfer approach to pH probes for intracellular imaging has considerable potential for modifications with other pH sensitive donors.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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