Substrate Catalysis Enhances Single-Enzyme Diffusion.
The article presents a study which measures the influence of substrate catalysis on the diffusion of urease enzyme with the use of Brownian dynamics simulations. Fluorescence correlation spectroscopy (FCS) was utilized to measure the diffusion coefficients and urease activity was inhibited by pretre...
| Publicado en: | Journal of the American Chemical Society Vol. 132; no. 7; pp. 2110 - 2112 |
|---|---|
| Autores principales: | , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
2/24/2010
|
| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=48616395&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 48616395 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 2/24/2010 vid: 132 iid: 7 pid: 997 pub: American Chemical Society artinfo: ui: 48616395 10.1021/ja908773a ppf: 2110 ppct: 2 formats: tig: atl: Substrate Catalysis Enhances Single-Enzyme Diffusion. aug: au: Muddana, Han S. Sengupta, Samudra Matlouk, Thomas E. Sen, Ayusman Butler, Peter J. affil: Department of Bioengineering, Pennsylvania State University, University Park, Pennsylvania 16802 Department of Chemistry,Pennsylvania State University, University Park, Pennsylvania 16802 su: Catalysis research Urease Enzymes Diffusion Fluorescence spectroscopy Simulation methods & models sug: subj: Catalysis research Urease Enzymes Diffusion Fluorescence spectroscopy Simulation methods & models ab: The article presents a study which measures the influence of substrate catalysis on the diffusion of urease enzyme with the use of Brownian dynamics simulations. Fluorescence correlation spectroscopy (FCS) was utilized to measure the diffusion coefficients and urease activity was inhibited by pretreatment of the enzyme with 1 mM pyrocatechol for two hours. Results show that the catalysis could alter the diffusion of an enzyme in the presence of a substrate. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2010 holdings: @attributes: islocal: N |
|---|