Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair.
We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/ tRNA pairs created in Escherichia coli can be used to expand the genetic code of Saccharornyces care visiae. In the process we have solved the key challenges of producing a functional tRNA in yeast and disco...
| Publicado en: | Journal of the American Chemical Society Vol. 132; no. 42; pp. 14819 - 14825 |
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| Autores principales: | , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
10/27/2010
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| Sumario: | We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/ tRNA pairs created in Escherichia coli can be used to expand the genetic code of Saccharornyces care visiae. In the process we have solved the key challenges of producing a functional tRNA in yeast and discovered a pyrrolysyl-tRNA synthetase/tRNA pair that is orthogonal in yeast. Using our approach we have incorporated an alkyne-containing amino acid for click chemistry, an important post-translationally modified amino acid and one of its analogs, a photocaged amino acid and a photo-cross-linking amino acid into proteins in yeast. Extensions of our approach will allow the growing list of useful amino acids that have been incorporated in E. coli with variant pyrrolysyl-tRNA synthetase/tRNA pairs to be site-specifically incorporated into proteins in yeast. |
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