Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair.
We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/ tRNA pairs created in Escherichia coli can be used to expand the genetic code of Saccharornyces care visiae. In the process we have solved the key challenges of producing a functional tRNA in yeast and disco...
| Publicado en: | Journal of the American Chemical Society Vol. 132; no. 42; pp. 14819 - 14825 |
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| Autores principales: | , , , |
| Formato: | Artículo |
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American Chemical Society
10/27/2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=54937159&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 54937159 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 10/27/2010 vid: 132 iid: 42 pid: 997 pub: American Chemical Society artinfo: ui: 54937159 10.1021/ja104609m ppf: 14819 ppct: 6 formats: tig: atl: Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair. aug: au: Hancock, Susan M. Uprety, Rajendra Deiters, Alexander Chin, Jason W. affil: Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, U.K. North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 su: Genetic code Amino acids Transfer RNA Biosynthesis Alkynes sug: subj: Genetic code Amino acids Transfer RNA Biosynthesis Alkynes ab: We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/ tRNA pairs created in Escherichia coli can be used to expand the genetic code of Saccharornyces care visiae. In the process we have solved the key challenges of producing a functional tRNA in yeast and discovered a pyrrolysyl-tRNA synthetase/tRNA pair that is orthogonal in yeast. Using our approach we have incorporated an alkyne-containing amino acid for click chemistry, an important post-translationally modified amino acid and one of its analogs, a photocaged amino acid and a photo-cross-linking amino acid into proteins in yeast. Extensions of our approach will allow the growing list of useful amino acids that have been incorporated in E. coli with variant pyrrolysyl-tRNA synthetase/tRNA pairs to be site-specifically incorporated into proteins in yeast. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2010 holdings: @attributes: islocal: N |
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