Super-resolution Microscopy of Lipid Bilayer Phases.
Sub-diffraction optical imaging with nanometer resolution of lipid phase-separated regions is reported. Merocyanine 540, a probe whose fluorescence is sensitive to the lipid phase, is combined with super-resolution imaging to distinguish the liquid- and gel-phase nanoscale domains of lipid bilayers...
| Publicado en: | Journal of the American Chemical Society Vol. 133; no. 13; pp. 4664 - 4668 |
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| Autores principales: | , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
4/6/2011
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| Sumario: | Sub-diffraction optical imaging with nanometer resolution of lipid phase-separated regions is reported. Merocyanine 540, a probe whose fluorescence is sensitive to the lipid phase, is combined with super-resolution imaging to distinguish the liquid- and gel-phase nanoscale domains of lipid bilayers supported on glass. The monomer-dimer equilibrium of MC540 in membranes is deemed responsible for the population difference of single-molecule fluorescence bursts in the different phase regions. The extension of this method to other binary or ternary lipid models or natural systems provides a promising new super-resolution strategy. |
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