Super-resolution Microscopy of Lipid Bilayer Phases.
Sub-diffraction optical imaging with nanometer resolution of lipid phase-separated regions is reported. Merocyanine 540, a probe whose fluorescence is sensitive to the lipid phase, is combined with super-resolution imaging to distinguish the liquid- and gel-phase nanoscale domains of lipid bilayers...
| Publicado en: | Journal of the American Chemical Society Vol. 133; no. 13; pp. 4664 - 4668 |
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| Autores principales: | , |
| Formato: | Artículo |
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American Chemical Society
4/6/2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=62807794&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 62807794 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 4/6/2011 vid: 133 iid: 13 pid: 997 pub: American Chemical Society artinfo: ui: 62807794 10.1021/ja1099193 ppf: 4664 ppct: 4 formats: tig: atl: Super-resolution Microscopy of Lipid Bilayer Phases. aug: au: Chinkuei Kuo Hochstrasser, Robin M. affil: Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, United States su: Nanoelectromechanical systems Lipids Diffraction patterns High resolution imaging Fluorescence Bilayer lipid membranes sug: subj: Nanoelectromechanical systems Lipids Diffraction patterns High resolution imaging Fluorescence Bilayer lipid membranes ab: Sub-diffraction optical imaging with nanometer resolution of lipid phase-separated regions is reported. Merocyanine 540, a probe whose fluorescence is sensitive to the lipid phase, is combined with super-resolution imaging to distinguish the liquid- and gel-phase nanoscale domains of lipid bilayers supported on glass. The monomer-dimer equilibrium of MC540 in membranes is deemed responsible for the population difference of single-molecule fluorescence bursts in the different phase regions. The extension of this method to other binary or ternary lipid models or natural systems provides a promising new super-resolution strategy. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2011 holdings: @attributes: islocal: N |
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