Conformational Flexibility of DNA.
Pulsed Electron-Electron Double Resonance (PELDOR) on double-stranded DNA (ds-DNA) was used to investigate the conformational flexibility of helical DNA. Stretching, twisting, and bending flexibility of ds-DNA was determined by incorporation of two rigid nitroxide spin labels into a series of 20 bas...
| Publicado en: | Journal of the American Chemical Society Vol. 133; no. 34; pp. 13375 - 13380 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
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American Chemical Society
8/31/2011
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=66622140&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 66622140 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 8/31/2011 vid: 133 iid: 34 pid: 997 pub: American Chemical Society artinfo: ui: 66622140 10.1021/ja201244u ppf: 13375 ppct: 5 formats: tig: atl: Conformational Flexibility of DNA. aug: au: Marko, Andriy Denysenkov, Vasyl Margraf, Dominik Cekan, Pavol Schiemann, Olav Sigurdsson, Snorri Th. Prisner, Thomas F. affil: Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt, Germany Science Institute, University of Iceland, Dunhaga 3, 107 Reykjavik, Iceland Centre for Biomolecular Sciences, Centre ofMagnetic Resonance, University of St Andrews, North Haugh, KY16 9ST St Andrews, U.K. su: DNA Nitroxides DYNAMO (Computer program language) Conformational analysis Spiral computed tomography Resonance Electrons sug: subj: DNA Nitroxides DYNAMO (Computer program language) Conformational analysis Spiral computed tomography Resonance Electrons ab: Pulsed Electron-Electron Double Resonance (PELDOR) on double-stranded DNA (ds-DNA) was used to investigate the conformational flexibility of helical DNA. Stretching, twisting, and bending flexibility of ds-DNA was determined by incorporation of two rigid nitroxide spin labels into a series of 20 base pair (bp) DNA duplexes. Orientation-selective PELDOR experiments performed at both X-band (9 GHz/0.3 T) and G-band (180 GHz/6.4 T) with spin label distances in the range of 2-4 nm allowed us to differentiate between different simple models of DNA dynaniics existing in the literature. All of our experimental results are in full agreement with a dynamic model for ds-DNA molecules, where stretching of the molecule leads to a slightly reduced radius of the helix induced by a cooperative twist-stretch coupling. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2011 holdings: @attributes: islocal: N |
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