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Multicomponent Reactions for de Novo Synthesis of BODIPY Probes: In Vivo Imaging of Phagocytic Macrophages.

Multicomponent reactions are excellent tools to generate complex structures with broad chemical diversity and fluorescent properties. Herein we describe the adaptation of the fluorescent BODIPY scaffold to multicomponent reaction chemistry with the synthesis of BODIPY adducts with high fluorescence...

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Publicado en:Journal of the American Chemical Society Vol. 135; no. 43; pp. 16018 - 16022
Autores principales: Vázquez-Romero, Ana, Kielland, Nicola, Arévalo, María J., Preciado, Sara, Mellanby, Richard J., Yi Feng, Lavilla, Rodolfo, Vendrell, Marc
Formato: Artículo
Publicado: American Chemical Society 10/30/2013
Materias:
Imaging systems
Chemical synthesis
Macrophage activation
Acidification
Dyes & dyeing
Fluorescent dyes
Acceso en línea:Ver este registro en EBSCOhost
Sumario:Multicomponent reactions are excellent tools to generate complex structures with broad chemical diversity and fluorescent properties. Herein we describe the adaptation of the fluorescent BODIPY scaffold to multicomponent reaction chemistry with the synthesis of BODIPY adducts with high fluorescence quantum yields and good cell permeability. From this library we identified one BODIPY derivative (PhagoGreen) as a low-pH sensing fluorescent probe that enabled imaging of phagosomal acidification in activated macrophages. The fluorescence emission of PhagoGreen was proportional to the degree of activation of macrophages and could be specifically blocked by bafilomycin A, an inhibitor of phagosomal acidification. PhagoGreen does not impair the normal functions of macrophages and can be used to image phagocytic macrophages in vivo.