Multicomponent Reactions for de Novo Synthesis of BODIPY Probes: In Vivo Imaging of Phagocytic Macrophages.
Multicomponent reactions are excellent tools to generate complex structures with broad chemical diversity and fluorescent properties. Herein we describe the adaptation of the fluorescent BODIPY scaffold to multicomponent reaction chemistry with the synthesis of BODIPY adducts with high fluorescence...
| Publicado en: | Journal of the American Chemical Society Vol. 135; no. 43; pp. 16018 - 16022 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
10/30/2013
|
| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=91957276&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 91957276 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 10/30/2013 vid: 135 iid: 43 pid: 997 pub: American Chemical Society artinfo: ui: 91957276 10.1021/ja408093p ppf: 16018 ppct: 4 formats: tig: atl: Multicomponent Reactions for de Novo Synthesis of BODIPY Probes: In Vivo Imaging of Phagocytic Macrophages. aug: au: Vázquez-Romero, Ana Kielland, Nicola Arévalo, María J. Preciado, Sara Mellanby, Richard J. Yi Feng Lavilla, Rodolfo Vendrell, Marc affil: Barcelona Science Park, Baldiri Reixac 10-12, 08028 Barcelona, Spain Escuela Politécnica, Universidad Extremadura, Avda. de la Universidad s/n, 10003 Cáceres, Spain MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom Laboratory of Organic Chemistry, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain su: Imaging systems Chemical synthesis Macrophage activation Acidification Dyes & dyeing Fluorescent dyes sug: subj: Imaging systems Chemical synthesis Macrophage activation Acidification Dyes & dyeing Fluorescent dyes ab: Multicomponent reactions are excellent tools to generate complex structures with broad chemical diversity and fluorescent properties. Herein we describe the adaptation of the fluorescent BODIPY scaffold to multicomponent reaction chemistry with the synthesis of BODIPY adducts with high fluorescence quantum yields and good cell permeability. From this library we identified one BODIPY derivative (PhagoGreen) as a low-pH sensing fluorescent probe that enabled imaging of phagosomal acidification in activated macrophages. The fluorescence emission of PhagoGreen was proportional to the degree of activation of macrophages and could be specifically blocked by bafilomycin A, an inhibitor of phagosomal acidification. PhagoGreen does not impair the normal functions of macrophages and can be used to image phagocytic macrophages in vivo. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2013 holdings: @attributes: islocal: N |
|---|