The Chemopreventive Effect of the Dietary Compound Kaempferol on the MCF-7 Human Breast Cancer Cell Line Is Dependent on Inhibition of Glucose Cellular Uptake.
Our aim was to investigate the effect of several dietary polyphenols on glucose uptake by breast cancer cells. Uptake of3H-deoxy-D-glucose (3H-DG) by MCF-7 cells was time-dependent, saturable, and inhibited by cytochalasin B plus phloridzin. In the short-term (26 min), myricetin, chrysin, genistein,...
| Publicado en: | Nutrition & Cancer Vol. 67; no. 3; pp. 504 - 514 |
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| Autores principales: | , , , , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Apr2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103782422&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103782422 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Apr2015 vid: 67 iid: 3 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 103782422 101869113 10.1080/01635581.2015.1002625 NLM25719685 103782422 ppf: 504 ppct: 10 formats: fmt: @attributes: type: P tig: atl: The Chemopreventive Effect of the Dietary Compound Kaempferol on the MCF-7 Human Breast Cancer Cell Line Is Dependent on Inhibition of Glucose Cellular Uptake. aug: au: Azevedo, Cláudia Correia-Branco, Ana Araújo, João R. Guimarães, João T. Keating, Elisa Martel, Fátima affil: Department of Biochemistry, Faculty of Medicine, University of Porto, Porto, Portugal sug: subj: Chemoprevention Dietary Supplements Breast Neoplasms Therapy Cell Physiology Blood Glucose Physiology Phenols Therapeutic Use Nutrition Breast Neoplasms Physiopathology Human Female Academic Medical Centers Molecular Biology Glucose Physiology Carrier Proteins Cell Culture Techniques Biological Transport Glucose Metabolism Statistics Female ab: Our aim was to investigate the effect of several dietary polyphenols on glucose uptake by breast cancer cells. Uptake of3H-deoxy-D-glucose (3H-DG) by MCF-7 cells was time-dependent, saturable, and inhibited by cytochalasin B plus phloridzin. In the short-term (26 min), myricetin, chrysin, genistein, resveratrol, kaempferol, and xanthohumol (10–100 µM) inhibited3H-DG uptake. Kaempferol was found to be the most potent inhibitor of3H-DG uptake [IC50of 4 µM (1.6–9.8)], behaving as a mixed-type inhibitor. In the long-term (24 h), kaempferol (30 µM) was also able to inhibit3H-DG uptake, associated with a 40% decrease in GLUT1 mRNA levels. Interestingly enough, kaempferol (100 µM) revealed antiproliferative (sulforhodamine B and3H-thymidine incorporation assays) and cytotoxic (extracellular lactate dehydrogenase activity determination) properties, which were mimicked by low extracellular (1 mM) glucose conditions and reversed by high extracellular (20 mM) glucose conditions. Finally, exposure of cells to kaempferol (30 µM) induced an increase in extracellular lactate levels over time (to 731 ± 32% of control after a 24 h exposure), due to inhibition of MCT1-mediated lactate cellular uptake. In conclusion, kaempferol potently inhibits glucose uptake by MCF-7 cells, apparently by decreasing GLUT1-mediated glucose uptake. The antiproliferative and cytotoxic effect of kaempferol in these cells appears to be dependent on this effect. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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