Consumption of a dark roast coffee decreases the level of spontaneous DNA strand breaks: a randomized controlled trial.
Purpose: Coffee consumption has been reported to decrease oxidative damage in peripheral white blood cells (WBC). However, effects on the level of spontaneous DNA strand breaks, a well established marker of health risk, have not been specifically reported yet. We analyzed the impact of consuming a d...
| Publicado en: | European Journal of Nutrition Vol. 54; no. 1; pp. 149 - 157 |
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| Autores principales: | , , , , , , |
| Formato: | research tables/charts randomized controlled trial Journal Article |
| Publicado: |
Springer Nature
Feb2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103878641&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103878641 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Feb2015 vid: 54 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 103878641 100575845 10.1007/s00394-014-0696-x NLM24740588 103878641 ppf: 149 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Consumption of a dark roast coffee decreases the level of spontaneous DNA strand breaks: a randomized controlled trial. aug: au: Bakuradze, T. Lang, R. Hofmann, T. Eisenbrand, G. Schipp, D. Galan, J. Richling, E. affil: Division of Food Chemistry, Toxicology, and Molecular Nutrition, Department of Chemistry, University of Kaiserslautern, Erwin-Schroedinger-Str. 52 67663 Kaiserslautern Germany sug: subj: Coffee Administration and Dosage DNA Physiology Leukocytes Physiology Human Germany Funding Source Randomized Controlled Trials Random Assignment Intervention Trials Male Men's Health Coffee Classification Pretest-Posttest Design Biological Assay Nutritional Assessment Questionnaires Body Weight Evaluation Young Adult Adult Middle Age Body Mass Index Evaluation Descriptive Statistics Prospective Studies Coffee Analysis Chemistry, Physical Chromatography, High Pressure Liquid Mass Spectrometry Two-Tailed Test T-Tests Paired T-Tests Analysis of Covariance Single-Blind Studies Antioxidants Signal Transduction Adult: 19-44 years Middle Aged: 45-64 years Male ab: Purpose: Coffee consumption has been reported to decrease oxidative damage in peripheral white blood cells (WBC). However, effects on the level of spontaneous DNA strand breaks, a well established marker of health risk, have not been specifically reported yet. We analyzed the impact of consuming a dark roast coffee blend on the level of spontaneous DNA strand breaks. Methods: Healthy men ( n = 84) were randomized to consume daily for 4 weeks either 750 ml of fresh coffee brew or 750 ml of water, subsequent to a run in washout phase of 4 weeks. The study coffee was a blend providing high amounts of both caffeoylquinic acids (10.18 ± 0.33 mg/g) and the roast product N-methylpyridinium (1.10 ± 0.05 mg/g). Before and after the coffee/water consumption phase, spontaneous strand breaks were determined by comet assay. Results: At baseline, both groups exhibited a similar level of spontaneous DNA strand breaks. In the intervention phase, spontaneous DNA strand breaks slightly increased in the control (water only) group whereas they significantly decreased in the coffee group, leading to a 27 % difference within both arms ( p = 0.0002). Food frequency questionnaires indicated no differences in the overall diet between groups, and mean body weight during the intervention phases remained stable. The consumption of the study coffee substantially lowered the level of spontaneous DNA strand breaks in WBC. Conclusion: We conclude that regular coffee consumption contributes to DNA integrity. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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