Alpha-lipoic acid preserves the structural and functional integrity of red blood cells by adjusting the redox disturbance and decreasing O-GlcNAc modifications of antioxidant enzymes and heat shock proteins in diabetic rats.
Purpose: The aim of this study was to investigate whether the daily administration of α-lipoic acid (LA) during 4 weeks prevents the redox disturbance in red blood cells (RBC) described in diabetes Methods: Multiple low-dose streptozotocin (STZ) diabetes was induced in rats by the administration of...
| Publicado en: | European Journal of Nutrition Vol. 51; no. 8; pp. 975 - 987 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104436712&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104436712 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Dec2012 vid: 51 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104436712 83371242 10.1007/s00394-011-0275-3 NLM22094580 104436712 ppf: 975 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Alpha-lipoic acid preserves the structural and functional integrity of red blood cells by adjusting the redox disturbance and decreasing O-GlcNAc modifications of antioxidant enzymes and heat shock proteins in diabetic rats. aug: au: Mirjana, Mihailović Jelena, Arambašić Aleksandra, Uskoković Svetlana, Dinić Nevena, Grdović Jelena, Marković Goran, Poznanović Melita, Vidaković affil: Institute for Biological Research Siniša Stanković, University of Belgrade, Bulevar despota Stefana 142 11060 Belgrade Serbia sug: subj: Erythrocytes Analysis Oxidation-Reduction Evaluation Reactive Oxygen Species Adverse Effects Diabetes Mellitus Chemically Induced Animal Studies Rats Funding Source Descriptive Statistics Data Analysis Software Electrophoresis Silver Staining Immunoblotting Analysis of Variance Iron Blood ab: Purpose: The aim of this study was to investigate whether the daily administration of α-lipoic acid (LA) during 4 weeks prevents the redox disturbance in red blood cells (RBC) described in diabetes Methods: Multiple low-dose streptozotocin (STZ) diabetes was induced in rats by the administration of 40 mg/kg STZ intraperitoneally (i.p.) for 5 consecutive days. LA was applied at a dose of 10 mg/kg i.p. for 4 weeks, starting from the last day of STZ administration. Results: The LA-treated diabetic rats exhibited a general systemic improvement, revealed as the near restoration of body weight and of essential biochemical parameters. The latter was displayed as decreased hyperglycemia, lower triglyceride levels and lower serum activities of alanine aminotransferases and aspartate aminotransferases that point to a general improvement of diabetes-linked organ 'lesions'. The LA-treated diabetic rats also exhibited significant alleviation of oxidative stress, manifested as decreased lipid peroxidation and lower glycation levels of serum proteins and hemoglobin, while the RBC exhibited increased activities of antioxidant enzymes and elevated levels of reduced glutathione. In RBC, this was accompanied by decreased post-translational glycosylation by O-bound β- N-acetylglucosamine (O-GlcNAc) of the antioxidant enzymes superoxide dismutase and catalase and of heat shock proteins HSP70 and HSP90. Conclusion: LA through its powerful antioxidant activity preserves the structural and functional integrity of RBC in diabetes. The RBC can then assume a more efficient role as the first line of systemic defense against diabetic complications arising from oxidative stress-induced damage of other tissues and organs. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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