Guanylin and uroguanylin are produced by mouse intestinal epithelial cells of columnar and secretory lineage.
Guanylin (GN) and uroguanylin (UGN), through activation of guanylyl cyclase C (GCC), serve to control intestinal fluid homeostasis. Both peptides are produced in the intestinal epithelium, but their cellular origin has not been fully charted. Using quantitative PCR and an improved in situ hybridizat...
| Publicado en: | Histochemistry & Cell Biology Vol. 146; no. 4; pp. 445 - 456 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Oct2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118355086&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118355086 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Oct2016 vid: 146 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 118355086 10.1007/s00418-016-1453-4 118355086 ppf: 445 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Guanylin and uroguanylin are produced by mouse intestinal epithelial cells of columnar and secretory lineage. aug: au: Ikpa, Pauline Sleddens, Hein Steinbrecher, Kris Peppelenbosch, Maikel Jonge, Hugo Smits, Ron Bijvelds, Marcel affil: Department of Gastroenterology and Hepatology , Erasmus MC University Medical Center , 3000 CA Rotterdam The Netherlands sug: ab: Guanylin (GN) and uroguanylin (UGN), through activation of guanylyl cyclase C (GCC), serve to control intestinal fluid homeostasis. Both peptides are produced in the intestinal epithelium, but their cellular origin has not been fully charted. Using quantitative PCR and an improved in situ hybridization technique (RNAscope), we have assessed the expression of GN ( Guca2a), UGN ( Guca2b), and GCC ( Gucy2c) in mouse intestine. In the crypts of Lieberkühn, expression of Guca2a and Guca2b was restricted to cells of secretory lineage, at the crypt's base, and to a region above, previously identified as a common origin of cellular differentiation. In this compartment, comparatively uniform levels of Guca2a and Guca2b expression were observed throughout the length of the gut. In contrast, Guca2a and Guca2b expression in the villus-surface region was more variable, and reflected the distinct, but overlapping expression pattern observed previously. Accordingly, in jejunum and ileum, Guca2a and Guca2b were abundantly expressed by enterocytes, whereas in colon only Guca2a transcript was found in the surface region. In duodenum, only low levels of Guca2b transcript were observed in columnar cells, and Guca2a expression was restricted entirely to cells of the secretory lineage. Gucy2c was shown to be expressed relatively uniformly along the rostrocaudal and crypt-villus axes and was also found in the duodenal glands. Our study reveals novel aspects of the cellular localization of the GCC signaling axis that, apart from its role in the regulation of fluid balance, link it to pH regulation, cell cycle control, and host defense. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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