Imaging Cell Surface Glycans with Bioorthogonal Chemical Reporters.

The article discusses a study that introduces two different chemical reporters into sialic acid and N-acetylgalactosamine residues and then simultaneously imaged their associated cell surface glycans with fluorescent probes. In this study, the two chemical reporters used are the ketone and the azide...

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Publicado en:Journal of the American Chemical Society Vol. 129; no. 27; pp. 8400 - 8402
Autores principales: Chang, Pamela V., Prescher, Jennifer A., Hangauer, Matthew J., Bertozzi, Carolyn R.
Formato: Artículo
Publicado: American Chemical Society 7/11/2007
Materias:
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 7/11/2007
      vid: 129
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      pub: American Chemical Society
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        10.1021/ja070238o
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        atl: Imaging Cell Surface Glycans with Bioorthogonal Chemical Reporters.
      aug:
        au:
          Chang, Pamela V.
          Prescher, Jennifer A.
          Hangauer, Matthew J.
          Bertozzi, Carolyn R.
        affil: Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94720
      su:
        Polysaccharides
        Azides
        Ketones
        Cell membranes
        Fluorescent probes
        Imaging systems
        Sialic acids
      sug:
        subj:
          Polysaccharides
          Azides
          Ketones
          Cell membranes
          Fluorescent probes
          Imaging systems
          Sialic acids
      ab: The article discusses a study that introduces two different chemical reporters into sialic acid and N-acetylgalactosamine residues and then simultaneously imaged their associated cell surface glycans with fluorescent probes. In this study, the two chemical reporters used are the ketone and the azide. Results reveal that glycan imaging can be realized using the bioorthogonal chemical reporter technique. This capability should enable monitoring of glycan expression and dynamics.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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          year: 2007
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