Fluorescent Ratiometric MFC Probe Sensitive to Early Stages of α-Synuclein Aggregation.

The article focuses on the sensor molecules consisting of covalent adducts of Ala-to-Cys mutants of the 140aa protein a-synuclein (AS) with thiol-reactive (maleimide) probe (MFC) based on 2-(2-furyl)-3-hydroxychromones (FC). It says that AS140-MFC is sensitive to the early and intermediate stages of...

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Publicado en:Journal of the American Chemical Society Vol. 132; no. 23; pp. 7860 - 7862
Autores principales: Yushchenko, Dmytro A., Fauerbach, Jonathan A., Thirunavukkuarasu, hyamala, Jares-Erijman, Elizabeth A., Jovin, Thomas M.
Formato: Artículo
Publicado: American Chemical Society 6/16/2010
Materias:
Acceso en línea:Ver este registro en EBSCOhost
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        atl: Fluorescent Ratiometric MFC Probe Sensitive to Early Stages of α-Synuclein Aggregation.
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        au:
          Yushchenko, Dmytro A.
          Fauerbach, Jonathan A.
          Thirunavukkuarasu, hyamala
          Jares-Erijman, Elizabeth A.
          Jovin, Thomas M.
        affil:
          Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
          Departamento de Qulmica Orgdnica, Facultad de Ciencias Exactas y Naturales, Uniuersidad de Buenos Aires, CIHIDECAR, CONICET, 1428 Buenos Aires, Argentina
      su:
        Thiols
        Clustering of particles
        Intermediates (Chemistry)
        Molecules
        Fire assay
        Sampling (Process)
        Protein synthesis
      sug:
        subj:
          Thiols
          Clustering of particles
          Intermediates (Chemistry)
          Molecules
          Fire assay
          Sampling (Process)
          Protein synthesis
      ab: The article focuses on the sensor molecules consisting of covalent adducts of Ala-to-Cys mutants of the 140aa protein a-synuclein (AS) with thiol-reactive (maleimide) probe (MFC) based on 2-(2-furyl)-3-hydroxychromones (FC). It says that AS140-MFC is sensitive to the early and intermediate stages of the overall reaction. Meanwhile, the inherently continuous nature of the aggregation assay of MFC probe provides more detailed multiparameter data than thioflavin T (THT)-based sampling protocols.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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