Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...

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Detalles Bibliográficos
Publicado en:	Journal of the American Chemical Society Vol. 133; no. 27; pp. 10368 - 10372
Autores principales:	Chia-Hung Chen, Sarkar, Aniruddh, Yong-Ak Song, Miller, Miles A., Sung Jae Kim, Griffith, Linda G., Lauffenburger, Douglas A., Jongyoon Han
Formato:	Artículo
Publicado:	American Chemical Society 7/13/2011
Materias:	Biomolecules Microfluidic devices Microreactors Metalloproteinases Metastasis
Acceso en línea:	Ver este registro en EBSCOhost

Descripción
Sumario:	We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.