Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...
| Publicado en: | Journal of the American Chemical Society Vol. 133; no. 27; pp. 10368 - 10372 |
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| Autores principales: | , , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
7/13/2011
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=64116834&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 64116834 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 7/13/2011 vid: 133 iid: 27 pid: 997 pub: American Chemical Society artinfo: ui: 64116834 10.1021/ja2036628 ppf: 10368 ppct: 4 formats: tig: atl: Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator. aug: au: Chia-Hung Chen Sarkar, Aniruddh Yong-Ak Song Miller, Miles A. Sung Jae Kim Griffith, Linda G. Lauffenburger, Douglas A. Jongyoon Han affil: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 36-841, 77 Massachusetts Avenue, Cambridge Massachusetts 02139, United States Department of Biological Engineering, Massachusetts Institute of Technology, 56-651, 77 Massachusetts Avenue, Cambridge Massachusetts 02139, United States su: Biomolecules Microfluidic devices Microreactors Metalloproteinases Metastasis sug: subj: Biomolecules Microfluidic devices Microreactors Metalloproteinases Metastasis ab: We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2011 holdings: @attributes: islocal: N |
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