Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...

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Bibliographic Details

Published in:	Journal of the American Chemical Society Vol. 133; no. 27; pp. 10368 - 10372
Main Authors:	Chia-Hung Chen, Sarkar, Aniruddh, Yong-Ak Song, Miller, Miles A., Sung Jae Kim, Griffith, Linda G., Lauffenburger, Douglas A., Jongyoon Han
Format:	Article
Published:	American Chemical Society 7/13/2011
Subjects:	Biomolecules Microfluidic devices Microreactors Metalloproteinases Metastasis
Online Access:	View this record in EBSCOhost