Probiotic Characteristics of Lactic Acid Bacteria Isolated From Sui Wu'u: A Traditional Food From Bajawa, West Flores, Indonesia.

The probiotic potential of lactic acid bacteria originating from traditional food will always be a concern for researchers, along with increasing public awareness of health. One source of LAB isolates with probiotic potential is traditional food. This study aims to investigate the probiotic potentia...

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Publicado en:Current Research in Nutrition & Food Science Vol. 11; no. 3; pp. 1074 - 1087
Autores principales: RUBAK, YULIANA TANDI, LALEL, HERIANUS J. D., EBENHAIZAR SANAM, MAXS URIAS, NALLE, RYAN PIETER
Formato: pictorial research tables/charts Journal Article
Publicado: Current Research in Nutrition & Food Science Dec2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Dec2023
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      pub: Current Research in Nutrition & Food Science
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        10.12944/CRNFSJ.11.3.13
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        atl: Probiotic Characteristics of Lactic Acid Bacteria Isolated From Sui Wu'u: A Traditional Food From Bajawa, West Flores, Indonesia.
      aug:
        au:
          RUBAK, YULIANA TANDI
          LALEL, HERIANUS J. D.
          EBENHAIZAR SANAM, MAXS URIAS
          NALLE, RYAN PIETER
        affil: Department of Agrotechnology, Faculty of Agriculture, Universitas Nusa Cendana, Kupang, East Nusa Tenggara 85228, Indonesia.
      sug:
        subj:
          Probiotics
          Lactobacillus Analysis
          Lactic Acid Analysis
          Food Indonesia
          Culture Indonesia
          Pork Meat
          Human
          Indonesia
          Fermentation
          Antiinfective Agents
          Drug Resistance, Microbial
          Sequence Analysis
          Data Analysis Software
          Descriptive Statistics
          Methicillin-Resistant Staphylococcus Aureus
          Funding Source
      ab: The probiotic potential of lactic acid bacteria originating from traditional food will always be a concern for researchers, along with increasing public awareness of health. One source of LAB isolates with probiotic potential is traditional food. This study aims to investigate the probiotic potential of lactic acid bacteria from Sui Wu'u, a traditional food-based pork originating from Bajawa, East Nusa Tenggara, Indonesia. Sui Wu'u was prepared based on method of the Bajawa community and was analyzed after being stored for six months. Total LAB, morphological, and phenotypic identification was carried out as well as testing the ability of isolates to ferment sugar. Probiotics potency was tested on resistance to low pH and gastric acid, resistance to pathogenic bacteria, and exposure to antibiotics. The results indicated that LAB isolated from Sui Wu'u were dominated by the coci form. The four selected isolates had resistance with a survival rate of 88.3%–96.88% when exposed to pH 2.5 and a survival rate of 98.69%–99.03% when exposed to bile salts at 0.3%. Moderate resistance was exhibited by all isolates against E. coli, S. typhimurium, and S. aureus, while no resistance was observed against B. cereus. All strains demonstrated sensitivity to tetracycline, ampicillin, and chloramphenicol but exhibited resistant to streptomycin. All four isolates were identified as Lactococcus Lactis. These results show that four strains of Sui Wu'u have demonstrated their potential as probiotics.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
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        Journal Article
      ougenre: Article
    language: English
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